diff --git a/mercurial/hgweb/hgweb_mod.py b/mercurial/hgweb/hgweb_mod.py
--- a/mercurial/hgweb/hgweb_mod.py
+++ b/mercurial/hgweb/hgweb_mod.py
@@ -28,6 +28,7 @@ from .. import (
     error,
     hg,
     hook,
+    profiling,
     repoview,
     templatefilters,
     templater,
@@ -305,8 +306,9 @@ class hgweb(object):
         should be using instances of this class as the WSGI application.
         """
         with self._obtainrepo() as repo:
-            for r in self._runwsgi(req, repo):
-                yield r
+            with profiling.maybeprofile(repo.ui):
+                for r in self._runwsgi(req, repo):
+                    yield r
 
     def _runwsgi(self, req, repo):
         rctx = requestcontext(self, repo)
diff --git a/mercurial/hgweb/hgwebdir_mod.py b/mercurial/hgweb/hgwebdir_mod.py
--- a/mercurial/hgweb/hgwebdir_mod.py
+++ b/mercurial/hgweb/hgwebdir_mod.py
@@ -31,6 +31,7 @@ from .. import (
     encoding,
     error,
     hg,
+    profiling,
     scmutil,
     templater,
     ui as uimod,
@@ -217,7 +218,9 @@ class hgwebdir(object):
         return False
 
     def run_wsgi(self, req):
-        return self._runwsgi(req)
+        with profiling.maybeprofile(self.ui):
+            for r in self._runwsgi(req):
+                yield r
 
     def _runwsgi(self, req):
         try:
diff --git a/tests/test-profile.t b/tests/test-profile.t
--- a/tests/test-profile.t
+++ b/tests/test-profile.t
@@ -31,4 +31,18 @@ test --profile
 
 #endif
 
+#if lsprof serve
+
+Profiling of HTTP requests works
+
+  $ hg --profile --config profiling.format=text --config profiling.output=../profile.log serve -d -p $HGPORT --pid-file ../hg.pid -A ../access.log
+  $ cat ../hg.pid >> $DAEMON_PIDS
+  $ hg -q clone -U http://localhost:$HGPORT ../clone
+
+A single profile is logged because file logging doesn't append
+  $ grep CallCount ../profile.log | wc -l
+  \s*1 (re)
+
+#endif
+
   $ cd ..